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Bolton called to testify in House impeachment inquiry (Oct. 30, 2019)

And then when it came time for the Senate Trial, the GOP (who still controlled the Senate at the time), refused to call any witnesses including Bolton, who purportedly offered to testify -

John Bolton says he'll testify in Senate impeachment trial if subpoenaed

And remember, the above happened while 45 was still in office.

After the acquittal, Bolton went on his book tour starting some time in June 2020, after a court denied 45's attempt to halt the book release.

So I expect that once Biden was elected and took office in 2021, they expanded an investigation given that Bolton DID work under 45 before he was fired and allegations related to the "quid pro quo" by 45, were apparently included in the book (and they probably discovered more, once they started researching Bolton's activities under 45).